Real-time quantitative PCR has been used previously to detect relative gene expression and amplification, 29, 30 quantitation of bacterial or viral pathogens, 31 as well as gene dose detection.

In real-time PCR, data collection starts as soon as target amplification is first detected, unlike in traditional PCR where data can be collected only after a certain number of PCR cycles are over.

Scientists develop a label-free method for detecting DNA amplification in real time based on refractive index changes in diffracted light.

The Bio-Rad Real-Time PCR application, Amplification Central, provides researchers performing PCR and qPCR with several helpful tools including tutorials, troubleshooting tips, and assay-specific ...

Seegene says that its Tagging Oligonucleotide Cleavage and Extension (TOCE) technology can enable multiplex assays to be run on existing real-time PCR instrumentation.

2.Operational Proficiency: PCR Reaction Setup: Participants will acquire practical skills in setting up PCR reactions, including primer design, template preparation, and reaction components.

The polymerase chain reaction -- A brief comparison between in vivo DNA replication and in vitro PCR amplification -- The PCR in practice -- The different types and varieties of nucleic acid target ...

Scientists optimize PCR to ensure successful amplification and analysis.

By doubling the multiplexing capacity of any existing real-time PCR instrument, MuDT™ will maximize the value of the installed base of real-time PCR instruments. MuDT™ technology will be unveiled at ...

10 Tips to Improve Your qPCR and RT-qPCR Results Get the best results from your real-time PCR assays with these tips.

Quantitative real-time PCR is a modified version that incorporates fluorescence labeling to cumulatively measure DNA amplification, rather than monitoring it at the end of the process, as in ...